| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Cryopreservation of semen from functional sex-reversed genotypic females of the rainbow trout, Oncorhynchus mykiss
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| Autores: |
Ninhaus-Silveira,Alexandre
Foresti,Fausto
Tabata,Yara Aiko
Rigolino,Marcos Guilherme
Veríssimo-Silveira,Rosicleire
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| Data: |
2006-01-01
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| Ano: |
2006
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| Palavras-chave: |
Oncorhynchus mykiss
Cryopreservation
Sex reversal
Gynogenesis
Semen
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| Resumo: |
Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences (P>0.05) among thawed semen groups (M = 73.1±11.5%; R = 67.2±23.6%; G = 64±5.8%), confirming that the freezing methodology used was efficient to cryopreserve semen of all three trout groups.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100009
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132006000100009
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.49 n.1 2006
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| Direitos: |
info:eu-repo/semantics/openAccess
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